BMBC168 is a shared platform for biochemistry, molecular biology and cell biology techniques in our department.
- Submit your project to the following address and put your group leader/project leader in copy.
What we know how to do for you (or can help you do yourself):
Molecular Biology:
- Molecular cloning (digestion/ligation, InFusion ligation, Gateway cloning)
- Sequencing
- DNA purification (Maxi prep etc)
- PCR (DNA amplification)
- Mutagenesis
- Genomic DNA preparation
- qPCR (quantitative PCR)
- Microarrays
- FISH
- constructions for CRISPR
Protein Purification:
- Protein expression systems: E. coli, yeast (S. cerevisiae), baculovirus (in insect cells–Sf9), mammalian cells (Flp-In system HEK293 cells), cell-free protein expression
- Protein purification from tissues (brain, muscle…)
- Affinity chromatography using tags (His, GST etc)
- FPLC purification with or without tags
- Mass spectrometry analysis of proteins
- Purification of membrane proteins
- Basic techniques: SDS-PAGE gels, native gels, protein concentration by Bradford, protein labeling with fluorescent markers (Alexa etc)
Cell Biology Techniques:
- Transient transfection
- Stable cell line production (electroporation, lipofectants reagents, lentiviral/retroviral infection)
- Lentiviral production, concentration, titration, storage
- Immunofluorescence
- Confocal microscopy
- Flow cytometry (analyse and cell sorting)
- CRISPR
- Mycoplasma test
- ELISA test (checking absence viruses)
- STR authentication
- Microfabrication