Microscopie Moléculaire des Membranes (MMM)

Ancien membres

Doctorant

Pierre-Frederic Fribourg.
PhD Thesis 2012-2009. Structural analysis of efflux ABC transporters by electron microscopy. MENRT fellow.

Publications
Dezi, M., Fribourg, P.F., Di Cicco, A., Jault, J.M., Chami, M., Lévy, D., 2011. Binding, reconstitution and 2D crystallization of membrane or soluble proteins onto functionalised lipid layer observed in situ by reflected light microscopy. J. Struct. Biol. 174, 307-314.
Dezi, M., Fribourg, P.F., Di Cicco, A., Arnaud, O., Marco, S., Falson, P., Di Pietro, A., Lévy, D., 2010. The multidrug resistance half-transporter ABCG2 is purified as a tetramer upon selective extraction from membranes. Biochim Biophys Acta. Biomembrane 1798, 2094-2101.
Fribourg, P.F., Chami, M., Sorzano, C.O., Gubellini, F., Marabini, R., Marco, S., Jault, J.M., Lévy, D., J Mol Biol. 2014 May 15;426(10):2059-69. Inward and outward facing conformations of a bacterial multidrug of an ABC exporter in a lipidic environment
Currently Project Manager in Robotics (Aldebaran Robotics, http://www.aldebaran-robotics.com/fr/)

Johan Busselez
PhD Thesis 2007-2003. MENRT fellow. Structural analysis of bacterial photosynthetic super complexes by electron microscopy

Publications
Busselez, J., Cottevieille, M., Cuniasse, P., Gubellini, F., Boisset, N., Levy, D., 2007. Structural basis for the PufX-mediated dimerization of bacterial photosynthetic core complexes. Structure 15, 1674-1683.
Gonçalves, R., Busselez, J., Lévy, D., Seguin, J., Scheuring, S., 2005. Membrane insertion of Rhodopseudomonas acidophila light harvesting complex 2 investigated by high resolution AFM. . J. Stuct. Biol. 149, 79-86.
Gubellini, F., Francia, F., Busselez, J., Venturoli, G., Levy, D., 2006. Functional and structural analysis of the photosynthetic apparatus of Rhodobacter veldkampii. Biochemistry 45, 10512-10520.
Scheuring, S., Busselez, J., Levy, D., 2005. Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopy. J Biol Chem 280, 1426-1431.
Scheuring, S., Francia, F., Busselez, J., Melandri, B.A., Rigaud, J.L., Levy, D., 2004. Structural role of PufX in the dimerization of the photosynthetic core complex of Rhodobacter sphaeroides. J Biol Chem 279, 3620-3626.

Currently Post-Doc in CNB-Biocomputing Unit Madrid. Team J.M.Carazo.

D. Teboul.
PhD thesis. First year. 2008.

Masters 2

Theresa Fontaine, 2014, University Cergy-Pontoise
Su Jin Paik, 2014, UPMC
Pauline Vaur. ESPCI Intership. April-June 2012
Pierre-Frederic Fribourg. Master 2 Feb-June 2008
Johan Busselez. Master 2 Feb-June 2003

Post-doc

Manuela Dezi. 2012-2009.

Publications
Dezi, M., Di Cicco, A., Bassereau, P., Levy, D., 2013. Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents. Proc Natl Acad Sci U S A 110, 7276-7281.
Dezi, M., Fribourg, P.F., Di Cicco, A., Jault, J.M., Chami, M., Lévy, D., 2011. Binding, reconstitution and 2D crystallization of membrane or soluble proteins onto functionalised lipid layer observed in situ by reflected light microscopy. J. Struct. Biol. 174, 307-314.
Dezi, M., Fribourg, P.F., Di Cicco, A., Arnaud, O., Marco, S., Falson, P., Di Pietro, A., Lévy, D., 2010. The multidrug resistance half-transporter ABCG2 is purified as a tetramer upon selective extraction from membranes. Biochim Biophys Acta. Biomembrane 1798, 2094-2101.
Galián, C., Manon, F., Dezi, M., Torres, C., Ebel, C., Lévy, D., Jault, J.M., 2011. Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2D crystallization. PlosOne 6, e19677.
Seantier*, B., Dezi*, M., Gubellini, F., Berquand, A., Godefroy, C., Dosset, P., Lévy, D., Milhiet, P.E., 2011. Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers. J. Biomol. Recognition 23, 1-6. * First co-authors.

Currently: University Research Scientist, Maitre De Conference.
Université Paris Descartes, Laboratoire de Cristallographie& RMN Biologiques


Francesca Gubellini
2003-2006

Publications
Berquand, A., Levy, D., Gubellini, F., Le Grimellec, C., Milhiet, P.E., 2007. Influence of calcium on direct incorporation of membrane proteins into in-plane lipid bilayer. Ultramicroscopy 107, 928-933.
Busselez, J., Cottevieille, M., Cuniasse, P., Gubellini, F., Boisset, N., Levy, D., 2007. Structural basis for the PufX-mediated dimerization of bacterial photosynthetic core complexes. Structure 15, 1674-1683.
Fribourg, P.F., Chami, M., Sorzano, C.O., Gubellini, F., Marabini, R., Marco, S., Jault, J.M., Lévy, D., submitted. Inward and outward facing conformations of a bacterial multidrug of an ABC exporter in a lipidic environment
Gubellini, F., Francia, F., Busselez, J., Venturoli, G., Levy, D., 2006. Functional and structural analysis of the photosynthetic apparatus of Rhodobacter veldkampii. Biochemistry 45, 10512-10520.
Gubellini, F., Francia, F., Turina, P., Levy, D., Venturoli, G., Melandri, B.A., 2007. Heterogeneity of photosynthetic membranes from Rhodobacter capsulatus: size dispersion and ATP synthase distribution. Biochim Biophys Acta 1767, 1340-1352.
Milhiet, P.E., Gubellini, F., Berquand, A., Dosset, P., Rigaud, J.L., Le Grimellec, C., Levy, D., 2006. High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer. Biophys J 91, 3268-3275.
Orelle, C., Gubellini, F., Durand, A., Marco, S., Levy, D., Gros, P., Di Pietro, A., Jault, J.M., 2008. Conformational change induced by ATP binding in the multidrug ATP-binding cassette transporter BmrA. Biochemistry 47, 2404-2412.
Piñol, R., Jia L., Gubellini, F., Lévy, D., Albouy, P.A., Keller, P., .A., C., Li, M.H., 2007. Self-Assembly of PEG-b-Liquid Crystal Polymer: The Role of Smectic Order in the Formation of Nanofibers. . Macromolecules 40, 5625-5627.
Seantier*, B., Dezi*, M., Gubellini, F., Berquand, A., Godefroy, C., Dosset, P., Lévy, D., Milhiet, P.E., 2011. Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers. J. Biomol. Recognition 23, 1-6. * First co-authors.
Yang, J., Pinol, R., Gubellini, F., Levy, D., Albouy, P.A., Keller, P., Li, M.H., 2006. Formation of polymer vesicles by liquid crystal amphiphilic block copolymers. Langmuir 22, 7907-7911.

Currently. Research Scientist, Team R. Fronzes. Institut Pasteur

Alexandre Berquand 2006-2007

Publications
Berquand, A., Levy, D., Gubellini, F., Le Grimellec, C., Milhiet, P.E., 2007. Influence of calcium on direct incorporation of membrane proteins into in-plane lipid bilayer. Ultramicroscopy 107, 928-933.
Milhiet, P.E., Gubellini, F., Berquand, A., Dosset, P., Rigaud, J.L., Le Grimellec, C., Levy, D., 2006. High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer. Biophys J 91, 3268-3275.
Seantier*, B., Dezi*, M., Gubellini, F., Berquand, A., Godefroy, C., Dosset, P., Lévy, D., Milhiet, P.E., 2011. Transfer on hydrophobic substrates and AFM imaging of membrane proteins reconstituted in planar lipid bilayers. J. Biomol. Recognition 23, 1-6. * First co-authors.

Currently: Life Science Application Scientist Bruker Nano GmbH

Assistant Ingénieur

Marie-Eve Chagot 2010-2011

Florent Vanoverschelde Licence Pro Biphotonique Université Paris Diderot. Oct 2013- Aout 2014