Membranes et fonctions cellulaires

Membres de l’équipe

Pulkit Aditya


Pulkit Aditya Equipe Bassereau +33 (0)1 56 24 64 62 Tél : Retour à la liste

I am working on Reconstitution of membrane scission by ESCRT-III complexes.
Summary of my PhD project:  Endosomal sorting complexes required for transport (ESCRT) proteins are involved in severing of narrow membrane necks in several processes like HIV virus release, exosomal and intra-endosomal budding (Multi-vesicular bodies formation) from membranes, cytokinesis (cell division), nuclear envelope reformation, biogenesis of micro-vesicles, neuron pruning, dendritic spine maintenance. It is an evolutionarily conserved protein machinery composed of ESCRT-0, ESCRT-I, ESCRT-II, ESCRT-III and VPS4. ESCRT-III complexes and VPS4 have been proposed to constitute the membrane fission machinery in these processes. The proteins of these complexes tend to assemble into polymer filaments. Recent findings point towards an affinity of ESCRT-III to positively curved membranes or with a negative Gaussian curvature. However, the underlying mechanisms are still unclear. Different physical models have been proposed to account for membrane constriction and cleavage but no hard-experimental proof of any of the models has yet been provided. The objective of the PhD project is to understand the mechanism of ESCRT-III and VPS4-catalysed membrane remodeling processes leading to membrane fission.