Membranes et fonctions cellulaires

Bassereau

Patricia Bassereau Chef d'équipe Tél :

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Lipid membranes exhibit non-trivial properties, particularly at spatial scales much larger than molecular sizes. A purely molecular description of the membranes is insufficient to arrive at a quantitative understanding of their function, and concepts at mesoscopic scales from soft matter and statistical physics are needed. Moreover, cell membranes involve a large number of proteins which can have some activity and which are inserted or interact with the membranes, which completely changes the physical description.

Our general objective is to contribute to a better understanding of biological membranes and their role in cells .

Bassereau-pictures

Bio-membranes models and cell membranes

Our goal is to understand the role of lipid membranes and associated proteins in essential cellular functions such as:

  •  Intracellular trafficking,
  • Endo / exocytosis,
  • Infection of cells,
  • The transmembrane transport of ions (« active membranes »),
  • And the diffusion of proteins.

For this, our group develops multidisciplinary approaches , largely based on synthetic biology, biomimetic systems and quantitative physical measurements .

We also study the mechanisms leading to the formation of filopods and the generation of force by these cellular structures, both on living cells and on in vitro systems.

We develop different model membrane systems, especially on the basis of giant unilamellar vesicles (GUV) and purified proteins (cytosolic or transmembrane). Our experiments generally combine quantitative confocal microscopy, optical clamps, micromanipulation, micropipette aspiration, as well as single particle tracking for diffusion measurements. As a large part of our projects concern deformations (curvature) and membrane mechanics, the group has developed experiments with membrane nanotubes drawn from GUVs or cells.

Our approaches and questions are motivated by our close collaboration with cell biologists at the Institut Curie and externally, with microbiologists and theorists .

Publications clés

Année de publication : 2016

Mijo Simunovic, Emma Evergren, Ivan Golushko, Coline Prévost, Henri-François Renard, Ludger Johannes, Harvey T McMahon, Vladimir Lorman, Gregory A Voth, Patricia Bassereau (2016 Oct 4)

How curvature-generating proteins build scaffolds on membrane nanotubes.

Proceedings of the National Academy of Sciences of the United States of America : 113 : DOI : 10.1073/pnas.1606943113

Année de publication : 2015

Coline Prévost, Hongxia Zhao, John Manzi, Emmanuel Lemichez, Pekka Lappalainen, Andrew Callan-Jones, Patricia Bassereau (2015 Feb 26)

IRSp53 senses negative membrane curvature and phase separates along membrane tubules.

Nature communications : 8529 : DOI : 10.1038/ncomms9529
Matthias Garten, Sophie Aimon, Patricia Bassereau, Gilman E S Toombes (2015 Feb 5)

Reconstitution of a transmembrane protein, the voltage-gated ion channel, KvAP, into giant unilamellar vesicles for microscopy and patch clamp studies.

Journal of visualized experiments : JoVE : 52281 : DOI : 10.3791/52281
Henri-François Renard, Mijo Simunovic, Joël Lemière, Emmanuel Boucrot, Maria Daniela Garcia-Castillo, Senthil Arumugam, Valérie Chambon, Christophe Lamaze, Christian Wunder, Anne K Kenworthy, Anne A Schmidt, Harvey T McMahon, Cécile Sykes, Patricia Bassereau, Ludger Johannes (2015 Jan 22)

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Nature : 493-6 : DOI : 10.1038/nature14064

Année de publication : 2014

Laura Picas, Julien Viaud, Kristine Schauer, Stefano Vanni, Karim Hnia, Vincent Fraisier, Aurélien Roux, Patricia Bassereau, Frédérique Gaits-Iacovoni, Bernard Payrastre, Jocelyn Laporte, Jean-Baptiste Manneville, Bruno Goud (2014 May 19)

BIN1/M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin.

Nature communications : 5647 : DOI : 10.1038/ncomms6647
Ayako Yamada, Alexandre Mamane, Jonathan Lee-Tin-Wah, Aurélie Di Cicco, Coline Prévost, Daniel Lévy, Jean-François Joanny, Evelyne Coudrier, Patricia Bassereau (2014 Apr 7)

Catch-bond behaviour facilitates membrane tubulation by non-processive myosin 1b.

Nature communications : 3624 : DOI : 10.1038/ncomms4624
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