Dynamique spatio-temporelle des cellules du système immunitaire

Publications de l’équipe

Année de publication : 2020

A J Lomakin, C J Cattin, D Cuvelier, Z Alraies, M Molina, G P F Nader, N Srivastava, P J Sáez, J M Garcia-Arcos, I Y Zhitnyak, A Bhargava, M K Driscoll, E S Welf, R Fiolka, R J Petrie, N S De Silva, J M González-Granado, N Manel, A M Lennon-Duménil, D J Müller, M Piel (2020 Oct 16)

The nucleus acts as a ruler tailoring cell responses to spatial constraints.

Science (New York, N.Y.) : DOI : eaba2894 En savoir plus
Résumé

The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. In this study, we found that cells measure the degree of spatial confinement by using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development.

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Aleksandra S Chikina, Francesca Nadalin, Mathieu Maurin, Mabel San-Roman, Thibault Thomas-Bonafos, Xin V Li, Sonia Lameiras, Sylvain Baulande, Sandrine Henri, Bernard Malissen, Livia Lacerda Mariano, Jorge Barbazan, J Magarian Blander, Iliyan D Iliev, Danijela Matic Vignjevic, Ana-Maria Lennon-Duménil (2020 Sep 24)

Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption.

Cell : 411-428.e16 : DOI : S0092-8674(20)31090-4 En savoir plus
Résumé

The colon is primarily responsible for absorbing fluids. It contains a large number of microorganisms including fungi, which are enriched in its distal segment. The colonic mucosa must therefore tightly regulate fluid influx to control absorption of fungal metabolites, which can be toxic to epithelial cells and lead to barrier dysfunction. How this is achieved remains unknown. Here, we describe a mechanism by which the innate immune system allows rapid quality check of absorbed fluids to avoid intoxication of colonocytes. This mechanism relies on a population of distal colon macrophages that are equipped with « balloon-like » protrusions (BLPs) inserted in the epithelium, which sample absorbed fluids. In the absence of macrophages or BLPs, epithelial cells keep absorbing fluids containing fungal products, leading to their death and subsequent loss of epithelial barrier integrity. These results reveal an unexpected and essential role of macrophages in the maintenance of colon-microbiota interactions in homeostasis. VIDEO ABSTRACT.

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Anita Kumari, Judith Pineau, Ana-Maria Lennon-Duménil, Martial Balland, Paolo Pierobon (2020 Aug 11)

Traction Force Microscopy to Study B Lymphocyte Activation.

Journal of visualized experiments : JoVE : DOI : 10.3791/60947 En savoir plus
Résumé

Traction force microscopy (TFM) enables the measurement of forces produced by a cell on a substrate. This technique infers traction force measurements from an experimentally observed displacement field produced by a cell pulling on an elastic substrate. Here, we adapted TFM to investigate the spatial and temporal structure of the force field exerted by B cells when activated by antigen engagement of the B cell receptor. Gel rigidity, bead density, and protein functionalization must be optimized for the study of relatively small cells (~ 6 µm) that interact with, and respond specifically to ligands for cell surface receptors.

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Yessia Hidalgo, Sarah Núñez, Maria Jose Fuenzalida, Felipe Flores-Santibáñez, Pablo J Sáez, Jessica Dorner, Ana-Maria Lennon-Dumenil, Victor Martínez, Emmanuel Zorn, Mario Rosemblatt, Daniela Sauma, Maria Rosa Bono (2020 May 16)

Thymic B Cells Promote Germinal Center-Like Structures and the Expansion of Follicular Helper T Cells in Lupus-Prone Mice.

Frontiers in immunology : 696 : DOI : 10.3389/fimmu.2020.00696 En savoir plus
Résumé

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the activation of autoreactive T and B cells, autoantibody production, and immune complex deposition in various organs. Previous evidence showed abnormal accumulation of B cells in the thymus of lupus-prone mice, but the role of this population in the progression of the disease remains mostly undefined. Here we analyzed the spatial distribution, function, and properties of this thymic B cell population in the BWF1 murine model of SLE. We found that in diseased animals, thymic B cells proliferate, and cluster in structures that resemble ectopic germinal centers. Moreover, we detected antibody-secreting cells in the thymus of diseased-BWF1 mice that produce anti-dsDNA IgG autoantibodies. We also found that thymic B cells from diseased-BWF1 mice induced the differentiation of thymocytes to follicular helper T cells (T). These data suggest that the accumulation of B cells in the thymus of BWF1 mice results in the formation of germinal center-like structures and the expansion of a T population, which may, in turn, activate and differentiate B cells into autoreactive plasma cells. Therefore, the thymus emerges as an important niche that supports the maintenance of the pathogenic humoral response in the development of murine SLE.

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Hélène D Moreau, Ana-Maria Lennon-Duménil, Paolo Pierobon (2020 Mar 4)

« If you please… draw me a cell ». Insights from immune cells.

Journal of cell science : DOI : jcs244806 En savoir plus
Résumé

Studies in recent years have shed light on the particular features of cytoskeleton dynamics in immune cells, challenging the classical picture drawn from typical adherent cell lines. New mechanisms linking the dynamics of the membrane-cytoskeleton interface to the mechanical properties of immune cells have been uncovered and shown to be essential for immune surveillance functions. In this Essay, we discuss these features, and propose immune cells as a new playground for cell biologists who try to understand how cells adapt to different microenvironments to fulfil their functions efficiently.

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Sandra Sofía Edwards-Jorquera, Floris Bosveld, Yohanns A Bellaïche, Ana-María Lennon-Duménil, Álvaro Glavic (2020 Jan 16)

Trpml controls actomyosin contractility and couples migration to phagocytosis in fly macrophages.

The Journal of cell biology : DOI : e201905228 En savoir plus
Résumé

Phagocytes use their actomyosin cytoskeleton to migrate as well as to probe their environment by phagocytosis or macropinocytosis. Although migration and extracellular material uptake have been shown to be coupled in some immune cells, the mechanisms involved in such coupling are largely unknown. By combining time-lapse imaging with genetics, we here identify the lysosomal Ca2+ channel Trpml as an essential player in the coupling of cell locomotion and phagocytosis in hemocytes, the Drosophila macrophage-like immune cells. Trpml is needed for both hemocyte migration and phagocytic processing at distinct subcellular localizations: Trpml regulates hemocyte migration by controlling actomyosin contractility at the cell rear, whereas its role in phagocytic processing lies near the phagocytic cup in a myosin-independent fashion. We further highlight that Vamp7 also regulates phagocytic processing and locomotion but uses pathways distinct from those of Trpml. Our results suggest that multiple mechanisms may have emerged during evolution to couple phagocytic processing to cell migration and facilitate space exploration by immune cells.

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Année de publication : 2019

Stankevicins L, Ecker N, Terriac E, Maiuri P, Schoppmeyer R, Vargas P, Lennon-Duménil AM, Piel M, Qu B, Hoth M, Kruse K, Lautenschläger F. (2020 Jan 14)

Deterministic actin waves as generators of cell polarization cues.

Proceedings of the National Academy of Sciences : 117 : Proc Natl Acad Sci U S A. 2020 Jan 14;117(2):826-835. doi: 10.1073/pnas.1907845117. Epub 2019 Dec 27. : 826,835 : DOI : 10.1073/pnas.1907845117 En savoir plus
Résumé

Dendritic cells « patrol » the human body to detect pathogens. In their search, dendritic cells perform a random walk by amoeboid migration. The efficiency of pathogen detection depends on the properties of the random walk. It is not known how the dendritic cells control these properties. Here, we quantify dendritic cell migration under well-defined 2-dimensional confinement and in a 3-dimensional collagen matrix through recording their long-term trajectories. We find 2 different migration states: persistent migration, during which the dendritic cells move along curved paths, and diffusive migration, which is characterized by successive sharp turns. These states exhibit differences in the actin distributions. Our theoretical and experimental analyses indicate that this kind of motion can be generated by spontaneous actin polymerization waves that contribute to dendritic cell polarization and migration. The relative distributions of persistent and diffusive migration can be changed by modification of the molecular actin filament nucleation and assembly rates. Thus, dendritic cells can control their migration patterns and adapt to specific environments. Our study offers an additional perspective on how dendritic cells tune their searches for pathogens.

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Anita Kumari, Judith Pineau, Pablo J Sáez, Mathieu Maurin, Danielle Lankar, Mabel San Roman, Katharina Hennig, Vanessa F Boura, Raphael Voituriez, Mikael C I Karlsson, Martial Balland, Ana-Maria Lennon Dumenil, Paolo Pierobon (2019 Jun 30)

Actomyosin-driven force patterning controls endocytosis at the immune synapse.

Nature communications : 2870 : DOI : 10.1038/s41467-019-10751-7 En savoir plus
Résumé

An important channel of cell-to-cell communication is direct contact. The immune synapse is a paradigmatic example of such type of interaction: it forms upon engagement of antigen receptors in lymphocytes by antigen-presenting cells and allows the local exchange of molecules and information. Although mechanics has been shown to play an important role in this process, how forces organize and impact on synapse function is unknown. We find that mechanical forces are spatio-temporally patterned at the immune synapse: global pulsatile myosin II-driven tangential forces are observed at the synapse periphery while localised forces generated by invadosome-like F-actin protrusions are detected at its centre. Noticeably, we observe that these force-producing actin protrusions constitute the main site of antigen extraction and endocytosis and require myosin II contractility to form. The interplay between global and local forces dictated by the organization of the actomyosin cytoskeleton therefore controls endocytosis at the immune synapse.

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Juan José Sáez, Jheimmy Diaz, Jorge Ibañez, Juan Pablo Bozo, Fernanda Cabrera Reyes, Martina Alamo, François-Xavier Gobert, Dorian Obino, María Rosa Bono, Ana-María Lennon-Duménil, Charles Yeaman, María-Isabel Yuseff (2019 Jun 15)

The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.

The Journal of cell biology : 2247-2264 : DOI : 10.1083/jcb.201811131 En savoir plus
Résumé

B lymphocytes capture antigens from the surface of presenting cells by forming an immune synapse. Local secretion of lysosomes, which are guided to the synaptic membrane by centrosome repositioning, can facilitate the extraction of immobilized antigens. However, the molecular basis underlying their delivery to precise domains of the plasma membrane remains elusive. Here we show that microtubule stabilization, triggered by engagement of the B cell receptor, acts as a cue to release centrosome-associated Exo70, which is redistributed to the immune synapse. This process is coupled to the recruitment and activation of GEF-H1, which is required for assembly of the exocyst complex, used to promote tethering and fusion of lysosomes at the immune synapse. B cells silenced for GEF-H1 or Exo70 display defective lysosome secretion, which results in impaired antigen extraction and presentation. Thus, centrosome repositioning coupled to changes in microtubule stability orchestrates the spatial-temporal distribution of the exocyst complex to promote polarized lysosome secretion at the immune synapse.

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Juan José Sáez, Ana-María Lennon-Duménil, María-Isabel Yuseff (2019 Jun 1)

Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes.

Methods in molecular biology (Clifton, N.J.) : 419-437 : DOI : 10.1007/978-1-4939-9450-2_29 En savoir plus
Résumé

The ability of B lymphocytes to capture external antigens (Ag) and present them as peptide fragments, loaded on major histocompatibility complex (MHC) class II molecules, to CD4 T cells is a crucial part of the adaptive immune response. This allows for T-B cooperation, a cellular communication that is required for B cells to develop into germinal centers (GC) and form mature high affinity antibody producing cells and to further develop B cell memory. MHC class II antigen presentation by B lymphocytes is a multistep process involving (1) Recognition and capture of external Ag by B lymphocytes through their B cell receptor (BCR), (2) Ag processing, which comprises the degradation of Ag in internal compartments within the B cell and loading of the corresponding peptide fragments on MHC class II molecules, and (3) Presentation of MHCII-peptide complexes to CD4 T cells. Here, we describe how to study the biochemical and morphological changes that occur in B lymphocytes at these three major levels.

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Lucie Barbier, Pablo J Sáez, Rafaele Attia, Ana-Maria Lennon-Duménil, Ido Lavi, Matthieu Piel, Pablo Vargas (2019 Apr 30)

Myosin II Activity Is Selectively Needed for Migration in Highly Confined Microenvironments in Mature Dendritic Cells.

Frontiers in immunology : 747 : DOI : 10.3389/fimmu.2019.00747 En savoir plus
Résumé

Upon infection, mature dendritic cells (mDCs) migrate from peripheral tissue to lymph nodes (LNs) to activate T lymphocytes and initiate the adaptive immune response. This fast and tightly regulated process is tuned by different microenvironmental factors, such as the physical properties of the tissue. Mechanistically, mDCs migration mostly relies on acto-myosin flow and contractility that depend on non-muscular Myosin IIA (MyoII) activity. However, the specific contribution of this molecular motor for mDCs navigation in complex microenvironments has yet to be fully established. Here, we identified a specific role of MyoII activity in the regulation of mDCs migration in highly confined microenvironments. Using microfluidic systems, we observed that during mDCs chemotaxis in 3D collagen gels under defined CCL21 gradients, MyoII activity was required to sustain their fast speed but not to orientate them toward the chemokine. Indeed, despite the fact that mDCs speed declined, these cells still migrated through the 3D gels, indicating that this molecular motor has a discrete function during their motility in this irregular microenvironment. Consistently, using microchannels of different sizes, we found that MyoII activity was essential to maintain fast cell speed specifically under strong confinement. Analysis of cell motility through micrometric holes further demonstrated that cell contractility facilitated mDCs passage only over very small gaps. Altogether, this work highlights that high contractility acts as an adaptation mechanism exhibited by mDCs to optimize their motility in restricted landscapes. Hence, MyoII activity ultimately facilitates their navigation in highly confined areas of structurally irregular tissues, contributing to the fine-tuning of their homing to LNs to initiate adaptive immune responses.

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Hélène D Moreau, Carles Blanch-Mercader, Rafaele Attia, Mathieu Maurin, Zahraa Alraies, Doriane Sanséau, Odile Malbec, Maria-Graciela Delgado, Philippe Bousso, Jean-François Joanny, Raphaël Voituriez, Matthieu Piel, Ana-Maria Lennon-Duménil (2019 Apr 16)

Macropinocytosis Overcomes Directional Bias in Dendritic Cells Due to Hydraulic Resistance and Facilitates Space Exploration.

Developmental cell : 171-188.e5 : DOI : S1534-5807(19)30235-7 En savoir plus
Résumé

The migration of immune cells can be guided by physical cues imposed by the environment, such as geometry, rigidity, or hydraulic resistance (HR). Neutrophils preferentially follow paths of least HR in vitro, a phenomenon known as barotaxis. The mechanisms and physiological relevance of barotaxis remain unclear. We show that barotaxis results from the amplification of a small force imbalance by the actomyosin cytoskeleton, resulting in biased directional choices. In immature dendritic cells (DCs), actomyosin is recruited to the cell front to build macropinosomes. These cells are therefore insensitive to HR, as macropinocytosis allows fluid transport across these cells. This may enhance their space exploration capacity in vivo. Conversely, mature DCs down-regulate macropinocytosis and are thus barotactic. Modeling suggests that HR may help guide these cells to lymph nodes where they initiate immune responses. Hence, DCs can either overcome or capitalize on the physical obstacles they encounter, helping their immune-surveillance function.

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Daisuke Inoue, Dorian Obino, Judith Pineau, Francesca Farina, Jérémie Gaillard, Christophe Guerin, Laurent Blanchoin, Ana-Maria Lennon-Duménil, Manuel Théry (2019 Mar 24)

Actin filaments regulate microtubule growth at the centrosome.

The EMBO journal : DOI : e99630 En savoir plus
Résumé

The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here, we show that increasing densities of actin filaments at the centrosome of lymphocytes are correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in disassembly of centrosomal actin and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. These two assays demonstrated that actin filaments constitute a physical barrier blocking elongation of nascent microtubules. Finally, we showed that cell adhesion and cell spreading lead to lower densities of centrosomal actin, thus resulting in higher microtubule growth. We therefore propose a novel mechanism, by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture.

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Année de publication : 2018

Dorian Obino, Luc Fetler, Andrea Soza, Odile Malbec, Juan José Saez, Mariana Labarca, Claudia Oyanadel, Felipe Del Valle Batalla, Nicolas Goles, Aleksandra Chikina, Danielle Lankar, Fabián Segovia-Miranda, Camille Garcia, Thibaut Léger, Alfonso Gonzalez, Marion Espéli, Ana-Maria Lennon-Duménil, Maria-Isabel Yuseff (2018 Dec 13)

Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse.

Cell reports : 3110-3122.e6 : DOI : S2211-1247(18)31815-1 En savoir plus
Résumé

Complete activation of B cells relies on their capacity to extract tethered antigens from immune synapses by either exerting mechanical forces or promoting their proteolytic degradation through lysosome secretion. Whether antigen extraction can also be tuned by local cues originating from the lymphoid microenvironment has not been investigated. We here show that the expression of Galectin-8-a glycan-binding protein found in the extracellular milieu, which regulates interactions between cells and matrix proteins-is increased within lymph nodes under inflammatory conditions where it enhances B cell arrest phases upon antigen recognition in vivo and promotes synapse formation during BCR recognition of immobilized antigens. Galectin-8 triggers a faster recruitment and secretion of lysosomes toward the B cell-antigen contact site, resulting in efficient extraction of immobilized antigens through a proteolytic mechanism. Thus, extracellular cues can determine how B cells sense and extract tethered antigens and thereby tune B cell responses in vivo.

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Mirjana Weimershaus, François-Xavier Mauvais, Loredana Saveanu, Cézaire Adiko, Joël Babdor, Anastasia Abramova, Sebastian Montealegre, Myriam Lawand, Irini Evnouchidou, Katharina Julia Huber, Alexandra Chadt, Markus Zwick, Pablo Vargas, Michael Dussiot, Ana Maria Lennon-Dumenil, Thomas Brocker, Hadi Al-Hasani, Peter van Endert (2018 Sep 27)

Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.

Cell reports : 3568-3581 : DOI : S2211-1247(18)31312-3 En savoir plus
Résumé

Both cross-presentation of antigens by dendritic cells, a key pathway triggering T cell immunity and immune tolerance, and survival of several pathogens residing in intracellular vacuoles are intimately linked to delayed maturation of vesicles containing internalized antigens and microbes. However, how early endosome or phagosome identity is maintained is incompletely understood. We show that Toll-like receptor 4 (TLR4) and Fc receptor ligation induces interaction of the GTPase Rab14 with the kinesin KIF16b mediating plus-end-directed microtubule transport of endosomes. As a result, Rab14 recruitment to phagosomes delays their maturation and killing of an internalized pathogen. Enhancing anterograde transport by overexpressing Rab14, promoting the GTP-bound Rab14 state, or inhibiting retrograde transport upregulates cross-presentation. Conversely, reducing Rab14 expression, destabilizing Rab14 endosomes, and inhibiting anterograde microtubule transport by Kif16b knockdown compromise cross-presentation. Therefore, regulation of early endosome trafficking by innate immune signals is a critical parameter in cross-presentation by dendritic cells.

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