Autres actus

Publication : les dangers de la migration cellulaire 

Piel/Science

L’équipe « Biologie cellulaire systémique de la polarité et de la division (CNRS, IPGG, Institut Curie) » dirigée par Matthieu Piel, directeur de recherche au CNRS, a publié dans Science à propos du complexe protéique ESCRT III et de son rôle lors de l’ouverture de l’enveloppe nucléaire pendant la migration.

ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death

Science 24 Mar 2016, DOI : 10.1126
Raab, M. Gentili, H. de Belly, H. R. Thiam, P. Vargas, A. J. Jimenez, F. Lautenschlaeger, Raphaël Voituriez, A. M. Lennon-Duménil, N. Manel, M. Piel

« In eukaryotic cells, the nuclear envelope separates the genomic DNA from the cytoplasmic space and regulates protein trafficking between the two compartments. This barrier is only transiently dissolved during mitosis. Here we found that it also opened at high frequency in migrating mammalian cells during interphase, allowing nuclear proteins to leak out and cytoplasmic proteins to leak in. This transient opening was caused by nuclear deformation and was rapidly repaired in an ESCRT (endosomal sorting complexes required for transport)-dependent manner. DNA double strand breaks coincided with nuclear envelope opening events. As a consequence, survival of cells migrating through confining environments depended on efficient nuclear envelope and DNA repair machineries. Nuclear envelope opening in migrating leukocytes could potentially have important consequences for normal and pathological immune responses. »